The general requirements of the rule apply to all persons subject to the rule, i.e., shippers, receivers, loaders and carriers, at all times when they are engaged in the transportation of food.
However the specific requirements for carriers in 21 CFR 1.908(e) only apply to the carrier when the carrier and shipper have established a written agreement that the carrier is responsible, in whole or in part, for sanitary conditions during the transportation operation. (21 CFR 1.908(e))